International biosafety and biosecurity manuals and guidance on best biosafety practices and procedures: 

• World Health Organisation (WHO) Laboratory Biosafety Manual 4th Edition, 2020
• NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules, 2019
• CDC and NIH Biosafety in Microbiological and Biomedical Laboratories 6th Edition, 2020
• Advisory Committee on Dangerous Pathogens (ACDP) Approved list of biological agents 4th Edition, 2021
• Management and operation of microbiological containment laboratories ACDP, 2019
• Guidance for SAPO licence holders on the containment and control of specified animal pathogens, 2015
• Personal protective equipment World Health Organization, 2020
• WHO Guidance on regulations for the transport of infectious substances 2021-2022
• CDC/USDA Select agents and toxins list
• World Organisation for Animal Health - Terrestrial and aquatic animal diseases
• WHO Tuberculosis laboratory biosafety manual 2012

South African national biosafety and biosecurity legislation with links to the relevant government departments, compliance guidelines and application forms:

• SA Guidelines: Biosafety and biosecurity compliance in research, teaching and testing facilities in SA 

Department of Health (DOH)

• National Health Act (Act 61 of 2003)
• Regulations Section 68, National Health Act, Notice R178 (Registration of microbiological laboratories and the acquisition, importation, handling, maintenance and supply of human pathogens)
• Application form to register a microbiological laboratory for the use of human pathogen cultures
• Application form for authorisation to use, keep or handle cultures or preparations of microorganisms 

Associated legislation and guidelines

• SAHPRA Guideline for import and export of medicines
• SAHPRA and DoH Good Clinical Practice: Clinical Trial Guidelines 2020

Department of Agriculture, Land Reform and Rural Development (DALRRD)
Genetically modified organisms (GMOs)

• GMO Act (Act 15 of 1997)
• GMO Guidelines and policy, Application forms, Tariffs

Animal Health

• Animal Diseases Act (Act 35 of 1984)
• Laboratory / Facility Biosafety and Biosecurity approval procedure manuals, Biosafety level checklists
• Guidelines and application form for Research approval in terms of Section 20 of the Animal Diseases Act 
• SAVC Registration of veterinary facilities and authorisation of laboratory animal technicians

Department of Trade, Industry and Competition (DTIC)

• SA Council for the non-proliferation of weapons of mass destruction (NPWMD)
• Non-proliferation of weapons of mass destruction Act (Act 87 of 1993)
• NPWMD Regulations: Biological, Chemical, Missiles, Nuclear

Transport regulations

• Transportation of Dangerous Goods Regulations as set out in the National Road Traffic Act 93 of 1996
• SANS 10228 (2012): The identification and classification of dangerous goods for transport by road and rail modes
• WHO Guidance on regulations for the transport of infectious substances 2019-2020

Department of Forestry, Fisheries and the Enviroment (DFFE)

• National Environmental Management: Biodiversity Act (NEMBA), (Act 10 of 2004)
• NEMBA Invasive alien plant species regulations: Alien and invasive species list 2014
• NEMBA Threatened or Protected Species (TOPS) permit and registration application process 
• Cape Nature Scientific research permit - Western Cape
• Agricultural Pests Act (Act 36 of 1983)
• Regulations for Hazardous Biological Agents, Government notice R1887 of 2022 (SA OHS Act, 1993)

Guidance on biological risk assessments

• World Health Organisation (WHO) Laboratory Biosafety Manual 4th Edition, 2020, Section 2, p5
• Biological Risk Assessment: General Considerations for Laboratories - CDC
• ISO 35001:2019(en) Biorisk management for laboratories and other related organisations
• Guideline for biological risk assessment: cell cultures

Guidance on the development of effective biosafety standard operating procedures:

• Guideline for the development of a biosafety standard operating procedure (SOP) document for laboratory procedures where biohazardous or potentially biohazardous materials are handled

National and international literature on relevant biosafety and biosecurity topics:

• Animal Research Biosafety. Applied Biosafety. Sep 2018, 130-142
• Biosafety and biosecurity measures: management of biosafety level 3 facilities
• International Journal of Antimicrobial Agents 2010, 36S, S70–S74
• Degradation and Deactivation of Bacterial Antibiotic Resistance Genes during Exposure to Free Chlorine, Monochloramine, Chlorine Dioxide, Ozone, Ultraviolet Light, and Hydroxyl Radical. Environ. Sci. Technol. 2019, 53, 2013−2026
• Donning and doffing PPE CDC
• Guidance from the Scientific Advisory Committee on Genetic Modification (SAGM)
• Safe Handling and Management of Monoclonal Antibodies - NSW
• Safe Handling of Monoclonal Antibodies in Healthcare Settings - Simplivia
• Biological Risks and Laboratory-Acquired Infections: A Reality That Cannot be Ignored in Health Biotechnology. Front Bioeng Biotechnol. 2015; 3: 56
• Biological Toxins as the Potential Tools for Bioterrorism. Int. J. Mol. Sci. 2019, 20(5), 1181
• Occupational health and safety when handling frogs - OSU
• Biocide Use in the Antimicrobial Era: A Review. Molecules 2021, 26, 2276
• Investigation of biocidal efficacy of commercial disinfectants used in public, private and workplaces during the pandemic event of SARS‑CoV‑2. Scientific Reports (2022) 12:5468
• Biohazard levels and biosafety protection for Mycobacterium tuberculosis strains with different virulence. Biosafety and Health Volume 2, Issue 3, September 2020, 135-141
• Classification of chemicals - Workplace Hazardous Materials Information System 1988 CCOHS

International databases of pathogen safety data sheets:

• Biological agents - OSHA (USA) 
• Pathogen safety data sheets - Public Health Agency of Canada
• Agent information sheets - Boston University
• Working with toxins / venoms in the laboratory - risk assessment example - UQ 

International databases of cell line safety data sheets: 

• Cellosaurus - a knowledge resource on cell lines 
• ATCC - Search cell products
• The European Collection of Authenticated Cell Cultures (ECACC)

Guidance on general laboratory disinfectants and safety data sheets:

• Virkon S
• Biocide D
• F10SC
• The Antimicrobial Spectrum of Disinfectants - CFSPH

Guidance on safe use of general biosafety equipment:

• Guide to Biosafety and Biological Safety Cabinets - ESCO
• Fundamentals of working safely in a BSC - factors affecting BSC airflow - CDC
• Difference between a fume hood and a Biosafety Cabinet
• Autoclave positioning and installation
• SANS347 Basic user checklist for autoclaves
• Handling of hazardous material: Safe use of centrifuges (Eppendorf Handling Solutions)
• Safe air in laboratories - TROX Technik
• Designing HVAC systems for biosafety level requirements - Pharmaceutical Online

Guidance on biosafety laboratory waste management:

• Biological liquid waste disposal in the lab: Best Practices Integra     

UCT Waste collection service provider certificates: 

• BCL
• Waste-Mart